FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5520217
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19500.016.6336651
GTATAGA24700.015.3670471
CTAATAC23750.014.9660663
GTATATA36850.014.4204211
GCGCACT6800.014.2059111
TCCGCAC9600.014.01527264
TCTATAC23200.013.9821493
GTCTTAG13900.013.9017011
TATACAG27950.013.7052515
TAGACAG20550.013.60250955
CTAGACT12050.013.4599944
CTAGGAC9000.013.4202183
TACACAG18300.013.3891535
CATATAC28350.013.2680623
TATACTG31200.012.9412775
TAATACT35850.012.8025514
CGCACCT9750.012.7381466
TACACCG13300.012.7142365
CTTACAC13250.012.5014223
TAGGTAG12150.012.4974745
Produced by FastQC (version 0.11.2)