FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2159589
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG4350.019.8323654
GTATTAG8250.019.2411
GTATTAT14100.015.6633481
CATATAC11300.015.574553
TATTATA15500.015.3617662
CGACCCG1851.02209364E-414.91871416
CTATACT9900.014.6398934
TATTAGG7850.014.506682
GCAGCGT6100.014.1427521
ACCGCGC2202.812051E-514.1140978
TAGGTAG5150.014.0716765
CGCACCT4202.7284841E-1113.96409366
TATACTG12650.013.9127655
CTAATAC8250.013.8033263
CCGTGCA3304.1314706E-813.5907229
TCTATAC10200.013.5326733
GTATAGA9700.013.5187211
TAATACT13100.013.4345354
CTAGACA6450.013.3753154
TATACCG5750.013.2034875
Produced by FastQC (version 0.11.2)