FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6926941
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG27750.016.9118791
GCGTCAG15000.016.3341124
TATTAGG25450.015.3215762
CGCACCT13600.015.22035666
TAGGTAG16750.015.0400435
TCCTTGC44200.014.2069988
TCCGCAC14850.014.17139864
CTAATAC31600.013.8687973
TAGTCAG17400.013.0865759
GTATCCT28850.012.9179581
GTATTAT51200.012.8729881
CCGCACC16450.012.58330365
GGTAGTC16950.012.4169047
CAGCGTC20350.012.0394532
ACTATGC31200.011.8326618
GCAGCGT21150.011.5841441
CGTCCGG7450.011.576920532
TAATACT47450.011.5635024
GTATATA53900.011.331841
ATCCTTG62200.011.3160027
Produced by FastQC (version 0.11.2)