FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8031142
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGC63900.012.09363769
GTATAGA26250.011.8306021
GTATTAG21700.011.7669951
GTATAAG28450.011.6434751
TATACTG32700.011.3973035
GTATATA28600.011.2204571
GTATATG25800.010.9669991
CCACGAG174000.010.88511369
GTATTAT38950.010.8080161
TATTAGG19450.010.6450982
ACGAGAC123950.010.35395369
TCCTATA17850.010.246032
GTATTAA24950.010.23422051
GTATAAT30300.010.1354031
GTATTAC25650.010.0894511
CTAATAC24750.010.0386493
AGACAGG87550.09.96951869
CGAGACA106400.09.92178169
TTATACT29600.09.9094744
TAGACAG26500.09.8967775
Produced by FastQC (version 0.11.2)