FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4472037
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG11800.017.2543954
GTATTAG18500.016.4137061
CTAGACT7950.015.6266214
GCAGCGT13250.015.3649761
TAGGTAG10200.015.2248075
CGCACCT7400.013.9861466
TATACTG28550.013.175285
GTATAAT26200.013.1702621
TAATACT32200.012.9675884
CTAATAC17850.012.952883
CAGCGTC15500.012.9129672
TCTATAC22250.012.5627513
ACATAGG10450.012.54500331
CCGCACC8550.012.50846265
GTATATA38550.012.1733541
TACACCG10550.012.102885
TCCGCAC8850.012.08444564
TATACGG15750.012.0509595
GTATAGA19000.011.9863251
TCCTAGG6100.011.88007452
Produced by FastQC (version 0.11.2)