FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5363292
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC11150.018.87399164
TAGGTAG14550.018.7358825
TAGTCAG12950.018.6490759
CGCACCT11750.018.49743366
GCGTCAG11700.017.990444
GTATTAG22100.017.9555761
CCGCACC13050.016.39041565
GGTAGTC14550.016.1246817
CTAATAC27200.015.9845173
TAATACT42600.015.4711154
TATTAGG22600.014.9627512
CTATGCA21750.014.2761889
ACTATGC26100.014.1443988
GTATAGA25450.013.829491
TATACAG32600.013.7605495
CCTAATA25950.013.6959972
CGCGCAT6050.013.68575440
ACATAGG16500.013.381531
GCAGCGT16050.013.1144051
ATAGGAG17150.013.07549133
Produced by FastQC (version 0.11.2)