FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736455
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14800.018.4200921
CGCACCT6650.015.04480466
TAGTCAG8400.014.3756679
TAGGTAG9450.014.2425685
GGTAGTC9550.014.0904137
GCGTCAG7600.014.0764124
TATTAGG15500.014.0258592
CTAGACT5750.013.8039664
GTATATA30750.013.46674251
CCCCTAG3851.5788828E-913.444881
TCCTATA13000.013.0068862
TATACAG20450.012.8255425
GCGCACT4852.3646862E-1112.80403111
CCGCACC7550.012.79426865
ACTGCGT7350.012.6747368
TCCGCAC7100.012.63337464
CTAATAC16800.012.5303853
ATTAGGT15600.012.3881743
GTATAAT23950.012.2472861
TAATACT24950.012.1718334
Produced by FastQC (version 0.11.2)