FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4248148
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG8950.015.809954
TAGGTAG9150.015.4647415
GTATTAG16850.014.745911
TCCTTGC23450.013.2420998
GTATAGA19400.012.807661
GTATATA38000.012.804851
TATACCG11250.012.2712095
GCAGCGT12500.012.1473531
CCTACAC8050.012.004023
TAATACT31050.011.7818614
TCCGCAC8200.011.77999664
CTATACT19950.011.7634754
CCTATAC14150.011.7071013
CCGCACC8050.011.57094665
CTAATAC19050.011.413273
TAGACAG14850.011.3880545
TACACCG9400.011.3818725
CGCACCT8250.011.29043866
GTATTAT30600.011.2776231
ATACCGT18900.011.1389136
Produced by FastQC (version 0.11.2)