FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4821063
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCT22450.016.2925971
TCCTTGC33300.016.0594148
GTATTAG19800.015.16194251
TATACAG25400.013.8573675
TAGGTAG11500.013.8030245
TCCGCAC9050.013.72363364
CGCACCT10050.013.38793966
TAATACT33200.013.1999084
GTATAGA21450.013.1912921
CTAATAC20900.012.8781543
CTCCTAT17150.012.8770581
TATTAGG18650.012.7667412
GGTAGTC12200.012.7262197
CCTTGCA21800.012.6605689
TATACTG28850.012.5590785
CCGCACC10450.012.54534265
CCTCCTA10950.012.2899821
CCCTTAG16850.012.2871771
CCTAATA23350.012.2659442
GACGCGC5359.094947E-1212.25209938
Produced by FastQC (version 0.11.2)