FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867763
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG7900.019.655764
GTATTAG15050.018.3422341
TAGGTAG9950.016.2996835
TATTAGG18150.014.639052
TCCTAAG10550.014.0641912
GCAGCGT10600.013.9978511
GGTAGTC10250.013.4637957
TCCTTGC24300.013.3460568
GTATAGA17350.013.3252111
TATACTG22450.013.2186325
CCTTGCG11350.013.0703479
GTATTAT30300.012.8686981
CGCACCT7050.012.7232766
TCCTACA14550.012.5693162
TAGTCAG10800.012.4582029
TATACAG20500.012.2877795
CCTAAGG8450.012.2509273
TTATACT24050.012.1957434
CAGCGTC12700.011.9549482
GTCCTAA9250.011.9373251
Produced by FastQC (version 0.11.2)