FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4911395
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22150.016.513421
GCGTCAG10350.015.0034224
CCCCTAG4950.013.9421481
GCAGCGT11650.013.625011
TAGGTAG12550.013.4736055
GTCCTAG7650.013.0810171
GTCCTAA10400.012.9400571
GGTAGTC11200.012.6300327
CAGCGTC12400.012.522892
TATACTG31000.012.1337825
CTAGACT7800.011.9450334
CTATACT23150.011.9249664
GTATATA55850.011.80089951
CCTACAC10200.011.50262453
GTATAGG16200.011.5022731
TAGTCAG11700.011.2052899
TCCTTGC30550.010.841738
TATTAGG24650.010.7792252
TATACAG27950.010.4946645
GTATATG33400.010.4346961
Produced by FastQC (version 0.11.2)