FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4170292
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG9800.019.0128214
CAGCGTC12250.015.491372
GCAGCGT12450.015.2426971
AGCGTCA13650.013.9028453
GTATTAG16700.013.016451
TATACAG24950.012.44695
TATACCG13450.012.3142585
ACCGCGC3104.1369876E-612.2427398
CATATAC28750.012.2415393
GTATATG26500.011.84851551
GTATTAC20100.011.50130751
TATACGG18450.011.4083475
GTCCTAA9400.011.3789531
GACCGCG3359.817368E-611.32910257
GTATATA48450.011.3232681
CTAGGAC6401.8189894E-1211.3217343
TATACTG29650.011.2885035
ACGGTGT15600.011.2795888
GTATTAT36100.011.278291
TACACTG14200.011.1778635
Produced by FastQC (version 0.11.2)