FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4187669
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG10150.017.339254
TAGGTAG9300.016.3266075
TCCTTGC27250.015.8261898
GTATTAG17350.015.3142841
CGCGCGG3001.180706E-814.94958154
GCAGCGT12300.014.027181
CCTTGCG12800.014.0157349
CAGCGTC12850.013.4269532
GTATCCT18550.013.2074781
GCGCGGG4002.8067006E-912.93713855
TCCTAAG11750.012.6281942
ATCCTTG35700.012.3701397
TAGTCAG9200.012.0000949
CCTACAC9800.011.9721983
AGCGTCA14250.011.8659423
TGTTAGG11350.011.8571242
TATTAGG17800.011.8255262
GTATATA37500.011.6863251
CGCGGGA3551.4701327E-611.66164556
CCTTGCA20000.011.557599
Produced by FastQC (version 0.11.2)