FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4821578
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTGC34500.017.0013778
GCGTCAG9600.016.894624
GTATTAG18850.015.559421
CCTTGCG15100.015.3080769
GTATCCT22850.014.0437331
TCCGCAC8650.013.16155164
TATACAG29700.013.013185
CTCCTAG8100.012.7797411
CCCTATA14350.012.7446462
CGCACCT9500.012.7103766
ATCCTTG45450.012.6775947
TAGGTAG11250.012.5763095
TACACCG11250.012.5763095
CTAATAC22150.012.4633163
TATACTG30350.012.3933755
CGCGCGG3651.5439036E-712.28726654
CCGCACC9000.012.26638865
TCAATCC25150.012.2116374
CAGCGTC13450.012.0581142
CAATCCT27650.011.9811345
Produced by FastQC (version 0.11.2)