FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4304737
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCT18750.016.1950191
GCGTCAG9650.015.73368554
TCCTTGC28800.014.8548648
CCCCCTA3959.094947E-1214.8508691
CCTTGCG13500.014.5665059
CCTCCTA8400.013.5560971
GTATTAG17600.013.5280891
ATCCTTG37200.013.4482137
GCAGCGT11350.013.0729211
TAGGTAG10050.013.04735855
GTATATG23650.012.9855061
ACCGCGC3508.9232344E-812.8148888
GTATATA37300.012.5814321
CCGCGTG2509.3417795E-512.4198619
CGCGGGA2902.8170327E-511.89628156
CGCACCT7300.011.81479966
CCCTTAG12150.011.6441451
CTATACT18750.011.59429554
TACTTCC21450.011.5827134
TCCGCAC7750.011.57393164
Produced by FastQC (version 0.11.2)