FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4577784
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG10400.019.2440954
TCCTTGC31600.015.940518
GCAGCGT12800.015.9050641
GTATTAG18050.015.8669791
GTATCCT19600.015.4924461
TAGGTAG11500.015.3029515
CCTTGCG14200.015.0636539
CTAGACT8500.013.8026614
GTCCTAA9250.013.802361
CAGCGTC14550.013.7546282
ATCCTTG38700.013.2834767
CTAATAC21000.012.4879853
GTATAGA19400.012.45058251
AGCGTCA16400.012.203443
TAGACCG11100.012.1239595
TGTTAGG12550.012.0974212
CGCGCGG3752.1938467E-711.95969354
CCTACAC9550.011.9236353
CTAGGAC7550.011.8829563
CCTAGAC11950.011.8389733
Produced by FastQC (version 0.11.2)