FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5340679
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCT25050.022.7296451
CGCGCGG5300.020.17866554
TCCTTGC42950.018.5561528
CGCGGGA5450.018.35727156
CCTTGCG17950.015.9524879
GCGTCAG10000.015.874154
CCGAGGG7550.015.53626864
CCTTGCA30250.015.396549
GTATCCC11550.015.2371811
CCGGAGC7550.015.07903740
GACGCGC6650.015.04482238
AACGCGC7450.014.81836552
ACGCGCG7750.014.689953
GTATTAG20000.014.6657861
CGTCCGG8500.014.61120832
CGCACCG8350.014.46079836
GCGCACC8900.014.34228435
ATCCTTG49800.014.2717427
GGATCCT18450.014.2145671
GCATCCT20950.014.1654651
Produced by FastQC (version 0.11.2)