FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5552283
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG4950.021.60534554
CGCGGGA4950.019.5146856
TCCTTGC37900.017.6603188
GTATCCT27000.016.7430081
TCCTAAG13700.015.8687252
CGTCCGG6300.014.78535732
TAGGTAG12450.014.6904935
AACGCGC7600.014.52596652
GTTCCTA15600.014.3785361
GTATTAG22000.013.8033961
CCCCCTA5450.013.2968491
TACGTCC11000.013.1760874
CCTTGCG17500.013.0113499
CCTCCTA12000.012.9406841
CCTTGCA28400.012.8766819
CTTCCTA24150.012.8603071
CCGCGCG4053.3778633E-912.7776999
GCGCGGG7600.012.71021955
CCCTATA16100.012.6458552
ACGCGCG8200.012.62153253
Produced by FastQC (version 0.11.2)