FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6283324
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCG12300.024.96285636
CGGGCCG13050.023.26384722
GTATCCT26450.021.7862831
ACGGGCC14100.021.77610821
CGTCCGG11400.021.48636232
CCGGAGC15050.020.40153740
CGCGCGG6950.020.35206254
CCGAGGG9500.020.33638864
GCGCACC15200.020.20020535
CACGGGC15100.020.10550920
TCACGGG16100.019.71384419
GTCCGGC13600.018.51796733
CGCGGGA7950.018.22600756
GCGAACC11600.018.14183623
CGAGGGA11200.017.55764465
GGGCCGT18050.017.01069823
GGCCGTC18850.016.654824
CCGGCTG15800.016.59456635
CCGTCCG15200.016.3417431
CCTTGCG23250.016.0262139
Produced by FastQC (version 0.11.2)