FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1654766
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG21250.1284169483781997RNA PCR Primer, Index 46 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG6950.033.25810269
CGCGCGG1251.2655619E-724.83933854
AAAGGGG9650.024.66773668
TACGTCC3950.022.7137454
GACGCGC1906.7484507E-1021.7888938
TGCCGTC13550.021.13227745
TATGCCG13300.020.49193443
ATGCCGT13250.020.04852144
GTATCCT7000.019.716141
CGTATGC13850.019.42908541
ACGTCCC4150.019.1245965
CGACGTA4150.019.1199745
GCCGTCT13900.019.11146
ATTATAG7650.018.9452513
GATTACG5100.018.942961
GCGAACC3100.018.91884823
CGCGGGA1651.8298142E-618.81767856
GTATGCC14600.018.6673142
TGACGCG1852.64552E-718.64815137
CGTCCGG2801.2732926E-1118.4816532
Produced by FastQC (version 0.11.2)