FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5811257
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGA5100.017.58764556
GCGTCAG11600.017.5522614
CGCGCGG5000.017.2494254
GTATCCT26650.016.8326131
TCCTTGC40800.015.8138748
CCTTGCG17000.015.0180499
ACGCGCG6250.014.35151953
GCGCGGG6400.014.01515555
GTATTAG26400.013.72431851
GCAGCGT16500.012.9662131
GTATAGA25100.012.9228871
CAGCGTC15550.012.871282
CCTTGCA27900.012.4895819
CTTACAC15650.012.3484593
TCCTATA25400.012.2273662
CCTATAC18800.011.7479253
AACGCGC7700.011.6489652
GGTAGTC15450.011.612867
TAGGTAG16050.011.6109845
CCCCTAG4803.3905962E-911.5022871
Produced by FastQC (version 0.11.2)