FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5988023
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG4650.018.54782754
GTATCCT27950.018.2717531
TCCTTGC47550.017.777428
GTATTAG24050.016.93041
GCGTCAG12150.015.6208574
CCTTGCG20800.015.2596659
CGCGGGA5450.014.55931656
GCAGCGT14200.014.3371881
CCTTGCA33100.014.0710439
CGTCCGG7450.012.96616132
GGATCCT18300.012.8220691
TAGGTAG13300.012.7135415
ACGCGCG7150.012.54518253
ATCCTTG59900.012.4416077
CGGTCAG9200.012.37473115
CTTACAC15750.012.2694733
CCTATAC20850.012.2474063
CTCCTAG9950.012.1379481
GTATCCC15400.012.0996651
AACGCGC7700.012.09713852
Produced by FastQC (version 0.11.2)