FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3605849
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT39550.017.88243969
GCGAATC20550.017.2919869
CACGAGC10900.016.77523869
AGGCGAA54850.015.85051169
GCGTCAG6850.015.6146634
AAGGCGA70150.013.18033669
TAAGGCG87250.013.16734369
TGGGGGG5900.012.8656645
GCGAATG11000.012.859097569
ACTAAGG130250.012.71401969
TCCTATA6850.012.5922962
GAGCCCC7900.012.22785669
GGGCGGT6500.012.2075312
GACTAAG153650.012.192327569
CCCTCGG4003.8888174E-812.0753428
CCCCTAT6050.011.9760441
TAGGTAG5501.6370905E-1111.9193455
CTAAGGC109350.011.70508369
AGACTAA189900.011.59084469
GTACTAG8150.011.4302471
Produced by FastQC (version 0.11.2)