FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307316
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCT14950.020.3110221
CGCGGGA2750.020.07207356
TCCTTGC25050.019.1451138
CGCGCGG3300.018.81756854
CGTCCGG4600.017.24943732
CCGTCCG5300.016.27305431
GACGCGC4900.016.19359438
CCTTGCG10050.016.135029
GTATTAG12250.015.7740141
GCGTCAG6250.015.4590024
GTCCGGC5200.015.25911833
CGCGCAT5050.015.0294440
ACGCGCA5050.015.0294439
ATCCTTG32750.014.5386887
GCGCGGG4301.8189894E-1214.44138955
CCTCCTA7900.014.4137551
TAGGTAG7500.014.2629855
CTTAAGG8500.014.2086413
TGACGCG5100.014.20563437
CCTACAC7650.013.9831083
Produced by FastQC (version 0.11.2)