FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6974386
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTGC40500.014.822528
CTCCTAG9750.014.15646651
TCCGCAC13000.014.06516464
CGCACCT14550.013.98947166
GTATTAG25100.013.6100881
CTAGACT12400.013.0791274
TATACGG22000.013.0184115
CCGCACC15650.013.00618665
TCCTATA24800.012.6614362
GTCCTAC8150.012.2783451
TCCTAAG20050.012.2190452
CTAATAC32300.012.1787233
TAGGTAG14800.012.1239525
CCTTGCA31700.012.0804259
CCTTGCG17850.011.9831779
GTCCTAA16100.011.7878961
ACCGCGC5602.3646862E-1111.7055948
CTAGGAC11550.011.6515073
CATATAC41400.011.5854773
CCTAGAC15200.011.57783
Produced by FastQC (version 0.11.2)