FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n01_mini02_partii_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n01_mini02_partii_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2853256
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTGC20650.019.2143888
CTCCTAG4500.018.404161
CCTCCTA5450.017.0956081
GTATCCT12750.017.0509131
CCTTGCG9850.016.1116479
TCCTAGG3703.6379788E-1215.8557682
CCCCTAA4950.015.33681
CCTTGCA15800.015.0664289
CGGCGCG1851.0224781E-414.91836838
CGCGCAT3252.1154847E-914.86099140
GACGCGC3051.46792445E-814.70437638
CTAGACT5200.014.6004774
TACTTCC14450.014.3295024
CCGGAGC3152.244633E-814.23757240
GCGAACC4700.013.94629523
ACTAGAC7150.013.5144913
GCGTCAG5150.013.4020284
CGCACCG2851.6134109E-613.31529936
GGCGAAC5750.013.19951422
ATCCTTG24400.013.150727
Produced by FastQC (version 0.11.2)