FastQCFastQC Report
Tue 16 Apr 2019
HKFW7AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFW7AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11822882
Sequences flagged as poor quality0
Sequence length110
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT11194279.468308996063735No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6539585.53129093227861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACATG1329650.098.5414353
ACATGAC1348450.097.444895
ATGACAT1371850.095.987927
TGACATC1368850.095.959198
TACATGA1364150.095.770484
GACATCA1378400.095.170099
GGCTACA1388200.094.747391
CATGACA1407000.093.4447566
GCTACAT1413300.092.689042
AGCAGTG172350.078.678152
GTTTGGT1518150.071.80616104
GGCTAGA8200.065.4978261
GTATCAA214100.062.8661889
AAGCAGT222500.061.5996631
GGTATCA242900.055.5151528
GCAGTGG249350.054.691473
GGCGACA23350.053.0801051
GGCTACG11800.052.517821
GTGGTAT271500.050.3982546
GCTAGAT9550.050.2672232
Produced by FastQC (version 0.11.5)