FastQCFastQC Report
Tue 16 Apr 2019
HKFW7AFXY_n02_TVC514.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFW7AFXY_n02_TVC514.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29612845
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT675620.22815099325985058No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC374670.12652279779264708No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1363600.088.637171
AGCAGTG1480950.081.451272
GCAGTGG1630500.073.9398963
CAGTGGT1753500.068.788824
GTGGTAT1755950.068.701616
AGTGGTA1757850.068.70135
GGTATCA1778700.067.5656368
GTATCAA1846800.065.144589
TGGTATC2052900.058.8374377
CAACGCA1752150.038.02073312-13
GCAGAGT1761050.037.98655316-17
ATCAACG1798100.037.0967710-11
CATGGGG1033950.036.34792324-25
TACATGG1844750.035.78102522-23
ACGCAGA1875650.035.6628514-15
GGGTCCG164900.035.14428-29
AGTACAT1947500.034.144220-21
TCAACGC1760100.034.1073812-13
CAGAGTA1781150.033.8619418-19
AACGCAG1801100.033.3988114-15
Produced by FastQC (version 0.11.5)