FastQCFastQC Report
Tue 16 Apr 2019
HKFW7AFXY_n02_TVC514_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFW7AFXY_n02_TVC514_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36213180
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT816720.22553114639476565No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC464620.12830135326419828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1657400.088.66191
AGCAGTG1798300.081.59452
GCAGTGG1983400.073.9406743
GTGGTAT2138100.068.631076
CAGTGGT2140150.068.536564
AGTGGTA2151800.068.242455
GGTATCA2172400.067.372738
GTATCAA2260100.064.740029
TGGTATC2500400.058.8488587
CAACGCA2136100.037.87747612-13
GCAGAGT2153550.037.7226716-17
ATCAACG2201500.036.81473510-11
CATGGGG1267850.035.82095324-25
TACATGG2246500.035.6792722-23
ACGCAGA2280700.035.56604414-15
GGGTCCG195300.035.0651228-29
TCAACGC2151300.033.94071612-13
AGTACAT2387100.033.85223820-21
CAGAGTA2176950.033.6829318-19
AACGCAG2191900.033.31085614-15
Produced by FastQC (version 0.11.5)