FastQCFastQC Report
Tue 16 Apr 2019
HKFW7AFXY_n02_TVC514_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFW7AFXY_n02_TVC514_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23671561
Sequences flagged as poor quality0
Sequence length110
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT545100.23027632186994343No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC290010.12251410035865401No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT942700.086.042181
AGCAGTG1009900.080.038022
GCAGTGG1102300.073.232493
GTGGTAT1190100.068.003656
CAGTGGT1196300.067.607744
GGTATCA1194850.067.419978
AGTGGTA1206000.067.206255
GTATCAA1259800.063.927579
TGGTATC1345500.060.2692957
CAACGCA1175950.037.8445412-13
GCAGAGT1185850.037.8070416-17
ATCAACG1222400.036.4724210-11
CATGGGG688150.036.08906624-25
TACATGG1246950.035.40406422-23
ACGCAGA1279150.034.93764514-15
GGGTCCG125650.034.6590628-29
TCAACGC1185750.033.8502912-13
CAGAGTA1206450.033.53240618-19
AACGCAG1208950.033.24370614-15
GAGTACA1233700.032.66529520-21
Produced by FastQC (version 0.11.5)