FastQCFastQC Report
Tue 16 Apr 2019
HKFW7AFXY_n02_TVC514_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFW7AFXY_n02_TVC514_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38039295
Sequences flagged as poor quality0
Sequence length110
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGTCCGGCGCCGTGGCGTAGT865540.22753839155010627No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGTAAATTTTATTAAGGTTTC486190.12781256855575268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1744200.088.6776051
AGCAGTG1886800.081.8151862
GCAGTGG2086500.073.887593
GTGGTAT2239100.069.014266
CAGTGGT2238950.068.9027564
AGTGGTA2255250.068.614575
GGTATCA2277400.067.652698
GTATCAA2373600.064.985279
TGGTATC2608900.059.2656757
CAACGCA2242550.037.96687312-13
GCAGAGT2256050.037.91139216-17
ATCAACG2312750.036.90215310-11
CATGGGG1330000.035.95726824-25
TACATGG2359550.035.8275222-23
ACGCAGA2387750.035.76915414-15
GGGTCCG206050.035.27984628-29
TCAACGC2260200.034.035412-13
AGTACAT2512450.033.8913820-21
CAGAGTA2281100.033.88199218-19
AACGCAG2299950.033.49464814-15
Produced by FastQC (version 0.11.5)