FastQCFastQC Report
Tue 16 Apr 2019
HKFW7AFXY_n02_TVC514SamBC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFW7AFXY_n02_TVC514SamBC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24853695
Sequences flagged as poor quality0
Sequence length110
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT2182746287.82381050383052No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1287250.5179310360089314No Hit
GCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTTG1019370.4101482697039615No Hit
GGCGACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT731640.2943787633991646No Hit
GGCCACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT583060.23459690802514477No Hit
GGCTACATGACATCAACCATATCAGCAAAGTGATACGGGTATTATTTTTG525240.21133276158736156No Hit
GGCTACATGACATCAACAATATCAGCAAAAGTGATACGGGTATTATTTTT410230.16505795214755795No Hit
GGCAACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT338730.13628959396178314No Hit
GGGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTT315790.12705957806273876No Hit
GGCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTG297590.11973672325181427No Hit
GGCTACATGACATAAACCATATCAGCAAAAGTGATACGGGTATTATTTTT275190.11072397886913797No Hit
GTCTACATGACATCAACCATATCAGCAAAAGTGATACGGGTATTATTTTT266370.10717521076845918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCAACT201.10983565E-5104.057441
GGCTTAC5350.0104.057441
GGCTCCT154.608761E-4104.057441
GGCTCAC201.10983565E-5104.057441
CTCCCGG201.1130136E-5103.997573
GGACTCA252.6970338E-7103.9967358
CCTTGAC154.61947E-4103.996735
GGCTACA23426000.0103.8810961
GGCTACT46900.0103.835571
GACATCG11900.0103.559779
ACGTGAC9750.0103.463425
CTCCATG15750.0103.3372653
GCTACAT23555100.0103.315292
CTACATG23575050.0103.291973
TGTCATC18800.0103.166978
GACTACA17350.0103.1578141
GGCTCAT11550.0103.156511
TACATGA23623450.0103.156094
ACATGAC23745300.0103.121685
CATGACA23764900.0103.066816
Produced by FastQC (version 0.11.5)