FastQCFastQC Report
Tue 16 Apr 2019
HKFW7AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFW7AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11822882
Sequences flagged as poor quality0
Sequence length28
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGG680150.5752827440889624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGACA22450.019.3042931
CGGACAG16100.019.122172
TCGGGAC12450.018.6417711
AGTTGAG18650.018.5081127
AGGTCGG15650.018.2664016
GATACGA14000.018.2167669
CTCGAGG23850.018.1711271
GGTCGGA11700.018.1335477
AACACGG15850.018.03247610
GACAGCC16650.017.96921
CCCATGA15550.017.8893742
GGCCAAC14400.017.86342210
CCCGGAA13550.017.8590151
CTGCGAG16050.017.7500171
CTGGCAG14950.017.731681
TATCCGC19300.017.7177312
AAGACGT10950.017.6722773
GAAGGGC10200.017.5703936
GCCGTCG12700.017.488065
CGATGGC13350.017.4674171
Produced by FastQC (version 0.11.5)