FastQCFastQC Report
Tue 16 Apr 2019
HKFW7AFXY_n01_TVC514.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFW7AFXY_n01_TVC514.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29612845
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA108300.019.0564381
GACGAGA49600.018.93761410
CTGGCAG93400.018.6081921
TCGACGA78400.018.4063158
CCATGAC88950.018.3871697
GAACACC102050.018.3709285
TCGGGAC75700.018.18991
AGGTCGG87700.018.1653376
CCGGAAC89100.018.1643752
AATGGCG73850.018.14035810
CCGGACA80750.018.0201261
GTCGACG72100.017.9857437
CGCTGAG82800.017.951453
CATCGGG94250.017.8564471
GGAACAC91050.017.835324
CGGACAG63400.017.7703592
AGAAAGA50950.017.6853734
ATCGGGG66250.017.5207252
GCCGTGA51400.017.5169731
AGTTGAG74650.017.5039657
Produced by FastQC (version 0.11.5)