FastQCFastQC Report
Tue 16 Apr 2019
HKFW7AFXY_n01_TVC514_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFW7AFXY_n01_TVC514_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36213180
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGAC91450.019.0778181
CCCGGAA132400.018.9137021
AGGTCGG107200.018.7302026
CTGGCAG110800.018.5674691
CCATGAC108950.018.3736047
TCGACGA94150.018.3411758
GACGAGA61250.018.33443310
CCGGAAC107150.018.3083722
CCGGACA97700.018.0939791
CGGACAG76300.018.068292
AATGGCG88750.017.9947610
CATCGGG120650.017.890951
GAACACC122800.017.8828415
CATGCTC177800.017.7677841
AGTTGAG90900.017.7507387
GGAACAC109300.017.7361814
ATCGGGG83550.017.7251192
CGCTGAG105200.017.7162593
GTCGACG89400.017.6794787
GAGACGC72950.017.565117
Produced by FastQC (version 0.11.5)