FastQCFastQC Report
Tue 16 Apr 2019
HKFW7AFXY_n01_TVC514_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFW7AFXY_n01_TVC514_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23671561
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA89950.019.1731971
GACGAGA42550.018.74099310
CCGGAAC72450.018.4813182
TCGGGAC60300.018.4709781
TCGACGA64100.018.3946258
CCGGACA65400.018.3937021
CTGGCAG74000.018.3233831
AATGGCG57050.018.23852210
CATCGGG75900.018.1547051
GAACACC85600.018.0579595
GCCGTGA43350.018.0258581
AGTTGAG61550.018.0130637
AGGTCGG67050.017.9344256
CCATGAC69250.017.9161387
CGGACAG52850.017.882532
CGCTGAG68850.017.8810183
ACCGAAA53150.017.8643932
CAGCCAG121950.017.6708551
GGAACAC75950.017.6145654
CACGAGC52200.017.598764
Produced by FastQC (version 0.11.5)