FastQCFastQC Report
Tue 16 Apr 2019
HKFW7AFXY_n01_TVC514_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFW7AFXY_n01_TVC514_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38039295
Sequences flagged as poor quality0
Sequence length28
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAA133050.019.12611
TCGGGAC97200.018.5821421
AGGTCGG108150.018.4941626
GACGAGA69000.018.39540310
CCATGAC113050.018.368967
CCGGAAC108850.018.3553282
TCGACGA102350.018.3549238
CTGGCAG116150.018.2227341
CATCGGG129850.018.13951
AATGGCG93650.018.11044510
GAACACC126650.018.09475
CCGGACA99150.017.9169581
ATCGGGG91350.017.885152
CATGCTC186000.017.7170871
GTCGACG99050.017.6894537
CAGCCAG200850.017.6182441
AGTTGAG93700.017.5490937
AGAAAGA65850.017.5261694
GCCGTGA67350.017.5190561
CGATGGC104450.017.4820141
Produced by FastQC (version 0.11.5)