FastQCFastQC Report
Tue 16 Apr 2019
HKFW7AFXY_n01_TVC514SamBC.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFW7AFXY_n01_TVC514SamBC.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24853695
Sequences flagged as poor quality0
Sequence length28
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGAGA128450.020.3249744
CGAGGAG128350.020.237993
CCGGACA145650.020.207281
CGTGATA145650.020.1392671
CTCGAGG162250.019.8968471
AAACCCA125750.019.8076741
CCCATGA100250.019.776762
GAAGGGC63900.019.644966
CTGCGAG115000.019.6302011
CGAAAGA79550.019.611124
CGGCCAA104150.019.5900949
TAACGAC108900.019.5068151
GGAGAAC82850.019.5025081
CGACCAA104900.019.3922314
GCCCAGA98300.019.3820841
CGGACAG105200.019.3795812
GCGAGGT89500.019.3731583
TCGAGGA134100.019.3627912
AATGGAA97350.019.3564071
GCCACAC126050.019.3416394
Produced by FastQC (version 0.11.5)