FastQCFastQC Report
Thu 25 Jan 2024
HKFT5BGXV_n01_HW40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFT5BGXV_n01_HW40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9507515
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT879970.9255520501413882TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTTT244510.2571755080060352TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCGCGTAT163290.17174834854323134TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCGCGTTT112760.11860091727438768TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)