FastQCFastQC Report
Thu 25 Jan 2024
HKFT5BGXV_n01_HW27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFT5BGXV_n01_HW27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12316207
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTGG757020.6146535211693015TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGTTG498030.4043696245118323TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTGG494370.40139793038554805TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGTTTG466990.3791670601184277TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGGGG330280.26816697705714104TruSeq Adapter, Index 8 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGGGGGGTG149850.12166895213761836TruSeq Adapter, Index 8 (97% over 42bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)