FastQCFastQC Report
Thu 25 Jan 2024
HKFT5BGXV_n01_HW26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFT5BGXV_n01_HW26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20134933
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTGG658010.32680019347469397TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGTTGG587130.2915976924283781TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGTTTG489960.24333828178122072TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGGGGGTTG442180.21960837912894968TruSeq Adapter, Index 7 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGGGGGGTGG327470.1626377400908163TruSeq Adapter, Index 7 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGGGGGGGGG238840.11861971430448762TruSeq Adapter, Index 7 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATGGGGGGTTG227940.11320623714019809TruSeq Adapter, Index 7 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)