FastQCFastQC Report
Thu 25 Jan 2024
HKFT5BGXV_n01_HW21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFT5BGXV_n01_HW21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26826142
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGGTGG285260.10633657273565464TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCGGGGTTGG283420.10565067462924783TruSeq Adapter, Index 2 (97% over 42bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC271770.10130789585770478TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)