FastQCFastQC Report
Fri 19 May 2017
HKFN7BGX2_n01_mb32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFN7BGX2_n01_mb32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38003428
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC1910760.5027862223376269TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC223200.040.76527444
TCGTATG219550.040.31061643
CTCGTAT223000.039.933542
TCTCGTA228150.039.2054941
TATCTCG233750.039.05719439
ATCTCGT236800.038.2568540
ACACGTC270850.036.76391213
CGTCTGA271050.036.65508716
ACGTCTG275350.036.11443315
GCTTATC271600.035.4694436
ATCGGAA289700.034.789882
CACGTCT288000.034.57362414
GATCGGA291950.034.2485161
TAGCTTA286350.033.99606334
CACACGT295400.033.8347612
ACTAGCT293000.033.45706632
GTCACTA296350.033.23492429
AGCTTAT296800.032.79901535
TCACTAG301900.032.58725730
TCGGAAG312550.032.2386473
Produced by FastQC (version 0.11.2)