FastQCFastQC Report
Fri 19 May 2017
HKFN7BGX2_n01_mb31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFN7BGX2_n01_mb31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37671394
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC10748172.85313837868596TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1176350.042.97494544
ATCGGAA1281950.041.0046842
TCGTATG1238850.040.7695643
CTCGTAT1224050.040.71800642
GATCGGA1283400.040.6913031
TCTCGTA1227050.040.52680641
ACGATCA1250950.040.37394332
ATCTCGT1229050.040.32503540
ACACGTC1282150.040.30095313
CGTCTGA1275550.040.20695516
CGATCAG1252850.040.17553733
TCACGAT1258800.040.1641630
TCGGAAG1302800.040.149953
ACGTCTG1281500.040.1248915
GTCACGA1266300.039.9767729
CACGATC1265050.039.94475631
CGGAAGA1308300.039.8851474
GATCTCG1244500.039.81911539
CACACGT1299800.039.79406412
CACGTCT1295600.039.78006414
Produced by FastQC (version 0.11.2)