FastQCFastQC Report
Fri 19 May 2017
HKFN7BGX2_n01_mb30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFN7BGX2_n01_mb30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42625106
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC8073511.894073882185771TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA1025650.042.23614516
GATCGGA1029750.042.2348141
ATCGGAA1042350.042.0693052
ACACGTC1034650.042.06248513
ACGTCTG1030900.042.04869515
CGTATGC919600.042.02689444
AATCTCG925000.041.85314639
TCGGAAG1047700.041.7927973
CGGAAGA1050900.041.682244
CACGTCT1048350.041.43897214
TCTCGTA918350.041.31485441
CTCGTAT915200.041.2577442
TCGTATG914750.041.2444243
GCACACG1059450.041.1440811
ATCTCGT938550.040.9067440
CACACGT1067800.040.81012312
TCGCGTA32850.039.51095641
GAACTCC1108150.038.95429221
GTCACAC1105000.038.8379229
TTGAATC1065350.038.33046336
Produced by FastQC (version 0.11.2)