FastQCFastQC Report
Fri 19 May 2017
HKFN7BGX2_n01_mb29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFN7BGX2_n01_mb29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39477204
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC4325761.0957614931391797TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC469750.042.15831444
CTCGTAT474850.041.3160442
TCGTATG477550.041.21153643
TCTCGTA475650.041.1999941
CGTCTGA499550.041.06642516
ACACGTC504450.040.82202513
ATCTCGT484100.040.50417740
ACGTCTG507350.040.44353515
CATCTCG485750.040.352839
ATCGGAA526250.039.6626662
GATCGGA522300.039.637631
CACGTCT519100.039.6210214
GCACACG527350.039.18696611
CGGAAGA532200.039.044724
TCGGAAG533300.039.0426143
CACACGT535200.038.59164412
CTTAGGC539300.037.2309133
TTAGGCA554950.036.1219234
ACTTAGG564400.035.88732
TAGGCAT556950.035.85790635
Produced by FastQC (version 0.11.2)