Basic Statistics
Measure | Value |
---|---|
Filename | HKFN7BGX2_n01_mb28.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 41762335 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC | 2698648 | 6.461918376929835 | TruSeq Adapter, Index 1 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 291515 | 0.0 | 43.162685 | 44 |
GATCGGA | 301670 | 0.0 | 42.82009 | 1 |
ATCGGAA | 303980 | 0.0 | 42.77974 | 2 |
TCGGAAG | 304105 | 0.0 | 42.65773 | 3 |
CGGAAGA | 304470 | 0.0 | 42.537174 | 4 |
ACACGTC | 302055 | 0.0 | 42.47677 | 13 |
CGTCTGA | 301290 | 0.0 | 42.466362 | 16 |
ACGTCTG | 302125 | 0.0 | 42.37443 | 15 |
CACGTCT | 303390 | 0.0 | 42.24748 | 14 |
GCACACG | 304440 | 0.0 | 42.207344 | 11 |
CACACGT | 304450 | 0.0 | 42.169357 | 12 |
CGATCTC | 291075 | 0.0 | 42.120853 | 38 |
CACGATC | 290505 | 0.0 | 42.104347 | 36 |
ACGATCT | 290810 | 0.0 | 42.1023 | 37 |
GATCTCG | 291685 | 0.0 | 42.087273 | 39 |
TCACGAT | 294445 | 0.0 | 42.036407 | 35 |
CATCACG | 297950 | 0.0 | 42.002037 | 33 |
ATCACGA | 296645 | 0.0 | 41.950897 | 34 |
CTCGTAT | 295720 | 0.0 | 41.950573 | 42 |
TCTCGTA | 294360 | 0.0 | 41.949482 | 41 |