FastQCFastQC Report
Fri 19 May 2017
HKFN7BGX2_n01_mb28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFN7BGX2_n01_mb28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41762335
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC26986486.461918376929835TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2915150.043.16268544
GATCGGA3016700.042.820091
ATCGGAA3039800.042.779742
TCGGAAG3041050.042.657733
CGGAAGA3044700.042.5371744
ACACGTC3020550.042.4767713
CGTCTGA3012900.042.46636216
ACGTCTG3021250.042.3744315
CACGTCT3033900.042.2474814
GCACACG3044400.042.20734411
CACACGT3044500.042.16935712
CGATCTC2910750.042.12085338
CACGATC2905050.042.10434736
ACGATCT2908100.042.102337
GATCTCG2916850.042.08727339
TCACGAT2944450.042.03640735
CATCACG2979500.042.00203733
ATCACGA2966450.041.95089734
CTCGTAT2957200.041.95057342
TCTCGTA2943600.041.94948241