FastQCFastQC Report
Fri 19 May 2017
HKFN7BGX2_n01_mb27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFN7BGX2_n01_mb27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38535179
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC6440251.6712651055805396TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC704300.042.22418644
CTCGTAT698350.041.9598842
TCTCGTA698250.041.92471741
TCGTATG709500.041.88659743
AATCTCG699550.041.8030539
CGTCTGA730800.041.75114416
ACGTCTG734250.041.5908715
ACACGTC736450.041.49197813
ATCTCGT707400.041.41079740
ATCGGAA747950.041.1086432
GATCGGA744050.041.0295521
CACGTCT750650.040.72563614
TCGGAAG757550.040.5369533
GCACACG758900.040.3304911
CGGAAGA763400.040.2116974
CACACGT765400.039.98250612
GTAATCT751300.038.9649837
TGTAATC771750.037.9669836
GAACTCC808250.037.70880521
TAATCTC777000.037.7010738
Produced by FastQC (version 0.11.2)