FastQCFastQC Report
Fri 19 May 2017
HKFN7BGX2_n01_mb26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFN7BGX2_n01_mb26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37464165
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC12169753.2483708098125237TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1340200.042.99180244
ACACGTC1414150.042.7938213
CGTCTGA1411800.042.76140616
CTCGTAT1322200.042.68670742
TCGTATG1327450.042.67697543
ACGTCTG1416100.042.6563615
TCTCGTA1324650.042.64755641
GATCGGA1409000.042.6332781
ATCGGAA1425650.042.512572
CATCTCG1342000.042.4506739
CACGTCT1424800.042.43570314
ATCTCGT1341050.042.41857540
TCGGAAG1430450.042.339733
GCACACG1432700.042.27779411
CACACGT1434700.042.20545612
CGGAAGA1437750.042.1260574
GATCATC1418850.041.27609636
GAACTCC1465650.041.04610421
CCAGATC1453750.040.85990533
AGTCACC1471200.040.70627228