Basic Statistics
Measure | Value |
---|---|
Filename | HKFN7BGX2_n01_mb26.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 37464165 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC | 1216975 | 3.2483708098125237 | TruSeq Adapter, Index 7 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGTATGC | 134020 | 0.0 | 42.991802 | 44 |
ACACGTC | 141415 | 0.0 | 42.79382 | 13 |
CGTCTGA | 141180 | 0.0 | 42.761406 | 16 |
CTCGTAT | 132220 | 0.0 | 42.686707 | 42 |
TCGTATG | 132745 | 0.0 | 42.676975 | 43 |
ACGTCTG | 141610 | 0.0 | 42.65636 | 15 |
TCTCGTA | 132465 | 0.0 | 42.647556 | 41 |
GATCGGA | 140900 | 0.0 | 42.633278 | 1 |
ATCGGAA | 142565 | 0.0 | 42.51257 | 2 |
CATCTCG | 134200 | 0.0 | 42.45067 | 39 |
CACGTCT | 142480 | 0.0 | 42.435703 | 14 |
ATCTCGT | 134105 | 0.0 | 42.418575 | 40 |
TCGGAAG | 143045 | 0.0 | 42.33973 | 3 |
GCACACG | 143270 | 0.0 | 42.277794 | 11 |
CACACGT | 143470 | 0.0 | 42.205456 | 12 |
CGGAAGA | 143775 | 0.0 | 42.126057 | 4 |
GATCATC | 141885 | 0.0 | 41.276096 | 36 |
GAACTCC | 146565 | 0.0 | 41.046104 | 21 |
CCAGATC | 145375 | 0.0 | 40.859905 | 33 |
AGTCACC | 147120 | 0.0 | 40.706272 | 28 |