FastQCFastQC Report
Fri 19 May 2017
HKFN7BGX2_n01_mb25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFN7BGX2_n01_mb25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32288495
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC18007085.57693382735863TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1941350.043.436551
ATCGGAA1957250.043.3270072
TCGGAAG1969100.043.0381853
CGTATGC1949250.042.89930744
CGCCAAT1943450.042.78176533
CGTCTGA1976450.042.76943216
ACACGTC1979700.042.76781513
ACGTCTG1978650.042.7306415
ACGCCAA1954600.042.65132532
GTCACGC1957650.042.6458929
CTCGTAT1953150.042.6455642
CGGAAGA1988150.042.6288574
TATCTCG1950600.042.62302839
TCGTATG1961650.042.6144143
TCTCGTA1954850.042.6071541
TCACGCC1961600.042.50710730
CACGTCT1991550.042.50213214
CACGCCA1962900.042.4821831
AGTCACG1970600.042.47760428
ATCTCGT1962900.042.44559540