Basic Statistics
Measure | Value |
---|---|
Filename | HKFN7BGX2_n01_mb25.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 32288495 |
Sequences flagged as poor quality | 0 |
Sequence length | 50 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC | 1800708 | 5.57693382735863 | TruSeq Adapter, Index 6 (100% over 50bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GATCGGA | 194135 | 0.0 | 43.43655 | 1 |
ATCGGAA | 195725 | 0.0 | 43.327007 | 2 |
TCGGAAG | 196910 | 0.0 | 43.038185 | 3 |
CGTATGC | 194925 | 0.0 | 42.899307 | 44 |
CGCCAAT | 194345 | 0.0 | 42.781765 | 33 |
CGTCTGA | 197645 | 0.0 | 42.769432 | 16 |
ACACGTC | 197970 | 0.0 | 42.767815 | 13 |
ACGTCTG | 197865 | 0.0 | 42.73064 | 15 |
ACGCCAA | 195460 | 0.0 | 42.651325 | 32 |
GTCACGC | 195765 | 0.0 | 42.64589 | 29 |
CTCGTAT | 195315 | 0.0 | 42.64556 | 42 |
CGGAAGA | 198815 | 0.0 | 42.628857 | 4 |
TATCTCG | 195060 | 0.0 | 42.623028 | 39 |
TCGTATG | 196165 | 0.0 | 42.61441 | 43 |
TCTCGTA | 195485 | 0.0 | 42.60715 | 41 |
TCACGCC | 196160 | 0.0 | 42.507107 | 30 |
CACGTCT | 199155 | 0.0 | 42.502132 | 14 |
CACGCCA | 196290 | 0.0 | 42.48218 | 31 |
AGTCACG | 197060 | 0.0 | 42.477604 | 28 |
ATCTCGT | 196290 | 0.0 | 42.445595 | 40 |